Work Schedule
Standard (Mon-Fri)
Environmental Conditions
Cold Room/Freezers -22degreesF/-6degrees C, Laboratory Setting, Office, Some degree of PPE (Personal Protective Equipment) required (safety glasses, gowning, gloves, lab coat, ear plugs etc.)
Key Responsibilities
- Lead the design, optimization, and validation of qPCR assays for gene expression, genotyping, and molecular diagnostics applications.
- Perform RNA/DNA extraction, cDNA synthesis, and nucleic acid quantification using spectrophotometric and fluorometric methods.
- Validate assay performance, including sensitivity, specificity, efficiency, and reproducibility.
- Analyze and interpret qPCR data using software such as Applied Biosystems QuantStudio, Bio-Rad CFX Manager, or equivalent platforms.
- Troubleshoot technical issues and propose innovative solutions to improve assay performance.
- Maintain accurate and detailed experimental records, laboratory notebooks, and data reports.
- Collaborate cross-functionally with R&D, QC, and bioinformatics teams to support product development or experimental studies.
- Ensure compliance with laboratory safety protocols, quality management systems, and regulatory standards (e.g., ISO, GLP, or CLIA, as applicable).
- Present experimental findings in technical meetings and contribute to scientific documentation and publications.
- Mentor junior scientists and research associates, providing technical guidance and fostering a culture of collaboration and continuous improvement.
Qualifications
Education: Master's or Ph.D. in Molecular Biology, Biochemistry, Genetics, or a related life sciences discipline. Experience requirements will vary based on degree.
Experience:
- Minimum of 25 years of hands-on experience performing qPCR (probe based chemistry preferred), including multiplexed qPCR reactions.
- Prior experience with assay development, validation, and data analysis.
- Familiarity with nucleic acid extraction instruments or workflows is expected.
- Additional experience in NGS is a plus.
Technical Skills:
- In-depth understanding of molecular biology principles, qPCR kinetics, and assay troubleshooting.
- Strong analytical and problem-solving skills with a data-driven mindset.
- Strong understanding of qPCR principles, primer/probe design, and amplification kinetics.
- Proficiency in data analysis, statistical interpretation, and report generation.
- Experience working under quality and regulatory standards preferred.
Soft Skills:
- Excellent organizational, communication, and problem-solving skills.
- Ability to work independently as well as collaboratively in a multidisciplinary environment.
- Strong attention to detail and scientific curiosity.
Preferred Qualifications
- Familiarity with bioinformatics tools for assay design
- Experience with digital PCR (dPCR)
- Knowledge of automation and data management systems.
