qPCR Scientist III, Molecular Biology

Key Responsibilities

• Lead the design, optimization, and validation of qPCR assays for gene expression, genotyping, and molecular diagnostics applications.

• Perform RNA/DNA extraction, cDNA synthesis, and nucleic acid quantification using spectrophotometric and fluorometric methods.

• Validate assay performance, including sensitivity, specificity, efficiency, and reproducibility.

• Analyze and interpret qPCR data using software such as Applied BiosystemsQuantStudio, Bio-Rad CFX Manager, or equivalent platforms.

• Troubleshoot technical issues and propose innovative solutions to improve assay performance.

• Maintainaccurateand detailed experimental records, laboratory notebooks, and data reports.

• Collaborate cross-functionally with R&D, QC, and bioinformatics teams to support product development or experimental studies.

• Ensure compliance with laboratory safety protocols, quality management systems, and regulatory standards (e.g., ISO, GLP, or CLIA, as applicable).

• Present experimental findings in technical meetings and contribute to scientific documentation and publications.

• Mentor junior scientists and research associates,providingtechnical guidance and fostering a culture of collaboration and continuous improvement.

Qualifications

Education:Master's or Ph.D. in Molecular Biology, Biochemistry, Genetics, ora relatedlife sciences discipline.Experience requirements will vary based ondegree.

Experience:

• Minimum of 2-5 years of hands-on experience performing qPCR (probe basedchemistrypreferred), including multiplexed qPCR reactions.

• Prior experience with assay development, validation, and data analysis.

• Familiarity with nucleic acid extraction instrumentsor workflows is expected.

• Additionalexperience in NGS is a plus.

Technical Skills:

• In-depth understanding of molecular biology principles, qPCR kinetics, and assay troubleshooting.

• Strong analytical and problem-solving skills with a data-driven mindset.

• Strong understanding of qPCR principles, primer/probe design, andamplificationkinetics.

• Proficiencyin data analysis, statistical interpretation, and report generation.

• Experience working under quality and regulatory standards preferred.

Soft Skills:

• Excellent organizational, communication, and problem-solving skills.

• Ability to work independently as well as collaboratively in a multidisciplinary environment.

• Strong attention to detail and scientific curiosity.

Preferred Qualifications

• Familiarity with bioinformatics tools forassay design

• Experience withdigital PCR (dPCR)

• Knowledge of automation and data management systems.