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qPCR Molecular Biologist, Scientist II

1-4 Years
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  • Posted 20 hours ago
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Job Description

Job Description :

Work Schedule

Standard (Mon-Fri)

Environmental Conditions

Cold Room/Freezers -22degreesF/-6degrees C, Laboratory Setting, Office, Some degree of PPE (Personal Protective Equipment) required (safety glasses, gowning, gloves, lab coat, ear plugs etc.)

Key Responsibilities

  • Design, develop, andoptimizeqPCR assays for gene expression, genotyping, copy number variation, and pathogen detection.

  • Plan and execute experiments independently, ensuring data quality, reproducibility, and technical excellence.

  • Perform nucleic acid extraction, cDNA synthesis, and quantification using standard molecular methods.

  • Validate assay performance (sensitivity, specificity, and efficiency) and analyze results using platforms such as Applied BiosystemsQuantStudio or Bio-Rad CFX Manager.

  • Troubleshoot technical issues and implement improvements to enhance assay performance.

  • Maintain detailed experimental records and prepare technical reports, SOPs, and validation documentation.

  • Collaborate with product development, bioinformatics, and assay design teams to integrate molecular workflows.

  • Ensure adherence to safety protocols, quality systems, and regulatory requirements (ISO, GLP, CLIA).

Qualifications

Education:Bachelor's orMaster's in Molecular Biology, Biochemistry, Genetics, ora relatedlife sciences discipline.Experiencerequirementswill vary based ondegree.

Experience:

  • Minimum of1-4years of hands-on experience performingqPCR.Experience requirements will vary based ondegree.

  • Prior experience with assay development, validation, and data analysis.

  • Familiarity with nucleic acid extraction instruments is a plus.

Technical Skills:

  • Understanding of molecular biology principles, qPCR kinetics, and assay troubleshooting.

  • Strong analytical and problem-solving skills with a data-driven mindset.

  • Strong understanding of qPCR principles, primer/probe design, andamplificationkinetics.

  • Proficiencyin data analysis, statistical interpretation, and report generation.

  • Experience working under quality and regulatory standards preferred.

Soft Skills:

  • Excellent organizational, communication, and problem-solving skills.

  • Ability to work independently as well as collaboratively in a multidisciplinary environment.

  • Strong attention to detail and scientific curiosity.

Preferred Qualifications

  • Familiarity with bioinformatics tools forassay design

  • Experience withNGS

  • Experience withdigital PCR (dPCR)

  • Experience with qPCR multiplexing

  • Knowledge of automation and data management systems.

More Info

About Company

Thermo Fisher Scientific Inc. is an American supplier of scientific instrumentation, reagents and consumables, and software services. Based in Waltham, Massachusetts, Thermo Fisher was formed through the merger of Thermo Electron and Fisher Scientific in 2006. Thermo Fisher Scientific has acquired other reagent, consumable, instrumentation, and service providers, including: Life Technologies Corporation (2013), Alfa Aesar (2015),Affymetrix (2016),FEI Company (2016), BD Advanced Bioprocessing (2018),and PPD (2021).
As of 2017, the company had a market capitalization of $21 billion and was a Fortune 500 company. Annual revenue in 2021 was US$39.21 billion.
In March 2020, Thermo Fisher Scientific received emergency use authorization from the FDA for a test for SARS-CoV-2 to help mitigate the COVID-19 pandemic.

Job ID: 135984643