Job Description
This project aims to study ciliopathies such as Primary Ciliary Dyskinesia (PCD) and Polycystic Kidney Disease (PKD). By utilising human stem cell-derived models, the research seeks to further characterise these genetic disorders and evaluate potential treatments via gene therapy approaches.
- Maintenance of human pluripotent stem cell lines and multi-step directed differentiation to generate lung and kidney tissues, including the use of 3D organoids, air-liquid interface (ALI) cultures, and Flow Cytometry (FACS) for the characterisation and isolation of specific cell populations.
- Proficiency in core molecular biology techniques including cloning and PCR-based analysis for genetic characterisation.
- Production, titration, and application of viral vectors for gene editing and stable expression within differentiated cell models.
- Characterisation of ciliary morphology and protein localisation using multi-channel immunofluorescence and high-resolution confocal microscopy.
Job Requirement
- Academic Background: PhD or Master's degree in Biomedical Sciences, Molecular Biology, Bioengineering, or a related life sciences discipline.
- Research Focus: Proven track record in stem cell biology or regenerative medicine is highly preferred.
- Professional Experience:For PhD holders: 0-3 years of postdoctoral experience
Technical Competencies
- Cell Culture Mastery: Extensive experience in maintaining human pluripotent stem cells (hPSCs) and performing directed differentiation.
- Tissue Engineering: Proficiency in 3D organoid culture and/or Air-Liquid Interface (ALI) models.
- Gene Editing & Virology: Hands-on experience with viral vector production (Lentivirus/AAV) and molecular cloning techniques.
- Analytical Techniques: Skilled in Flow Cytometry (FACS) for cell sorting and analysis.
