Work Schedule
Standard (Mon-Fri)
Environmental Conditions
Cold Room/Freezers -22degreesF/-6degrees C, Laboratory Setting, Office, Some degree of PPE (Personal Protective Equipment) required (safety glasses, gowning, gloves, lab coat, ear plugs etc.)
Key Responsibilities
- Design, develop, and optimize qPCR assays for gene expression, genotyping, copy number variation, and pathogen detection.
- Plan and execute experiments independently, ensuring data quality, reproducibility, and technical excellence.
- Perform nucleic acid extraction, cDNA synthesis, and quantification using standard molecular methods.
- Validate assay performance (sensitivity, specificity, and efficiency) and analyze results using platforms such as Applied Biosystems QuantStudio or Bio-Rad CFX Manager.
- Troubleshoot technical issues and implement improvements to enhance assay performance.
- Maintain detailed experimental records and prepare technical reports, SOPs, and validation documentation.
- Collaborate with product development, bioinformatics, and assay design teams to integrate molecular workflows.
- Ensure adherence to safety protocols, quality systems, and regulatory requirements (ISO, GLP, CLIA).
Qualifications
Education: Bachelor's or Master's in Molecular Biology, Biochemistry, Genetics, or a related life sciences discipline. Experience requirements will vary based on degree.
Experience:
- Minimum of 1-4 years of hands-on experience performing qPCR . Experience requirements will vary based on degree.
- Prior experience with assay development, validation, and data analysis.
- Familiarity with nucleic acid extraction instruments is a plus.
Technical Skills:
- Understanding of molecular biology principles, qPCR kinetics, and assay troubleshooting.
- Strong analytical and problem-solving skills with a data-driven mindset.
- Strong understanding of qPCR principles, primer/probe design, and amplification kinetics.
- Proficiency in data analysis, statistical interpretation, and report generation.
- Experience working under quality and regulatory standards preferred.
Soft Skills:
- Excellent organizational, communication, and problem-solving skills.
- Ability to work independently as well as collaboratively in a multidisciplinary environment.
- Strong attention to detail and scientific curiosity.
Preferred Qualifications
- Familiarity with bioinformatics tools for assay design
- Experience with NGS
- Experience with digital PCR (dPCR)
- Experience with qPCR multiplexing
- Knowledge of automation and data management systems.
